New PDF release: High-Pressure Microbiology

By Chris Michiels, Douglas H. Bartlett, Abram Aertsen

ISBN-10: 1555814239

ISBN-13: 9781555814236

* First quantity solely dedicated to the consequences of excessive strain on microorganisms * Brings jointly the high-pressure body structure of piezophiles and non-piezophiles * Spans the diversity from easy technology to sensible functions * presents primary perception for nutrition technologists utilizing excessive strain for foodstuff renovation * contains special fabric to demonstrate bacterial variations to severe high-pressure environments * presents a foundation for novel biotechnological functions utilizing excessive strain * Highlights the microbial wealth and ecological effect of the deep biosphere * accomplished resource of data on ecology, body structure, and genomics of deep sea micro organism

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New PDF release: High-Pressure Microbiology

* First quantity fullyyt dedicated to the consequences of excessive strain on microorganisms * Brings jointly the high-pressure body structure of piezophiles and non-piezophiles * Spans the diversity from simple technology to useful functions * offers basic perception for nutrition technologists using excessive strain for nutrition renovation * contains exact fabric to demonstrate bacterial variations to severe high-pressure environments * presents a foundation for novel biotechnological purposes utilizing excessive strain * Highlights the microbial wealth and ecological effect of the deep biosphere * finished resource of knowledge on ecology, body structure, and genomics of deep sea micro organism

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These maturation events are crucial to make the particle infectious. The mature particles were more unstable against pressure than the cleavage-defective particles (Fig. 3), which can be explained by the metastability elicited upon maturation (28). We also have evidence that the gamma subunit is released after a cycle of compression and decompression. A similar situation would occur with picornaviruses, with pressure-induced release of VP4 (Fig. 2). In both cases, pressure induces the formation of a noninfectious particle, apparently a fusion-active state of these nonenveloped viruses.

Johnson, and J. L. Silva. 1994. Differences in pressure stability of the three components of cowpea mosaic virus: implications for virus assembly and disassembly. Biochemistry 33:8339– 8346. 9. Da Poian, A. , A. C. Oliveira, and J. L. Silva. 1995. Cold denaturation of an icosahedral virus. The role of entropy in virus assembly. Biochemistry 34:2672–2677. 10. De Souza, P. , R. Tuma, P. E. Prevelige, J. L. Silva, and D. Foguel. 1999. Cavity defects in the procapsid of bacteriophage P22 and the mechanism of capsid maturation.

FHV is an RNA insect virus; it is nonenveloped and a member of the family Nodaviridae. It is composed of a bipartite single-stranded RNA genome packaged in an icosahedral capsid of 180 copies of an identical protein (alpha protein). A fundamental property of many animal viruses is the postassembly maturation required for infectivity. FHV is constructed as a provirion, which matures to an infectious virion by cleavage of alpha protein into beta and gamma subunits. We found that maturation cleavage leads into a metastable state, in which dissociation of the coat protein is coupled to unfolding.

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High-Pressure Microbiology by Chris Michiels, Douglas H. Bartlett, Abram Aertsen


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